Azospirillum brasilense is a bacterium colonizing plant roots that exerts beneficial effects on the growth of agricultural crops (Lerner, Castro-Sowinski, Valverde, Lerner, Dror, Okon & Burdman, 2009). Azospirillum brasilense is usually found in the plant rhizosphere of cultivated plants and agricultural land, as well as in grasses and cereals. Prefers plant roots instead of open soil. The polysaccharides of the bacterium play an important role in its interactions with plant roots. Colonizes the root surface of plants through attachment and anchoring. A. brasilense Sp7 carries several genes involved in the synthesis and export of cell surface polysaccharides. Azospirillum fixes nitrogen in the soil and produces various vitamins and phytohormones to promote agricultural production. It has properties against the development of bacterial diseases or promotes disease resistance on rice crops (Lerner, Castro-Sowinski, Valverde, Lerner, Dror, Okon & Burdman, 2009). Intensive use of chemicals to treat plant diseases leads to environmental problems and health risks. Biological control based on plant growth-promoting bacteria is the most reasonable approach for protecting crops from pathogens. Azospirillum brasilense fixes nitrogen in the soil and promotes overall crop production. Promotes disease resistance of rice crops and resistance to the development of bacterial diseases. The development of chemical synthesis provides access to the necessary tetrasaccharide on which natural sources depend (Mandal, Dhara & Misra, 2014). Biological agents that promote plant growth are increasingly becoming a method to improve agricultural production and minimize the risks of chemical fertility. The development of chemical syntheses... half of the article... Erde, Lerner, Dror, Okon & Burdman, 2009). The alteration of these genes meant that Azospirillum brasilense Sp7 did not produce biofilms as effectively. The alterations in the mutants were affected by their altered lipopolysaccharides and exopolysaccharides compared to the wild-type strain. Overall, these genes are involved in the biosynthesis of extracellular polysaccharides. Overall, tetrasaccharide synthesis was carried out as its 2-aminoethyl glycoside corresponded to the O-specific lipopolysaccharide of Azospirllum brasilense strain Sp7 to show why the development of chemical syntheses facilitated access to this necessary tetrasaccharide . After glycosylation, the use of thioglycosides as both donor and acceptor reduced the number of steps. This plant growth-promoting bacterium helps produce vitamins and hormones that promote agricultural production.